CD40 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32519
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32519-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the CD40 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human PBM cells with CD40 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD40 antibody.
2 / 3
IHC staining of FFPE human tonsil tissue with CD40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of human Raji lysate with CD40 antibody at 0.5ug/ml. Predicted molecular weight is 30-45 kDa depending on glycosylation level.
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Flow cytometry testing of human PBM cells with CD40 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD40 antibody.
IHC staining of FFPE human tonsil tissue with CD40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human Raji lysate with CD40 antibody at 0.5ug/ml. Predicted molecular weight is 30-45 kDa depending on glycosylation level.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,ELISA: 0.5-1ug/ml (human protein tested); request BSA-free format for coating
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. It is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids E21-R193 from the human protein were used as the immunogen for the CD40 antibody.
Limitation:
This CD40 antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P25942