CD40 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32520
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32520-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD40 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of mouse spleen lysate with CD40 antibody at 0.5ug/ml. Predicted molecular weight is 32-45 kDa depending on glycosylation level.
2 / 3
IHC testing of FFPE mouse spleen with CD40 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 3
Flow cytometry testing of human U937 cells with CD40 antibody at 1ug/10^6 cells (cells blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD40 antibody.
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Western blot testing of mouse spleen lysate with CD40 antibody at 0.5ug/ml. Predicted molecular weight is 32-45 kDa depending on glycosylation level.
IHC testing of FFPE mouse spleen with CD40 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U937 cells with CD40 antibody at 1ug/10^6 cells (cells blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD40 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,ELISA: 0.5-1ug/ml (mouse protein tested); request BSA-free format for coating
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. It is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids L20-R193 from the mouse protein were used as the immunogen for the CD40 antibody.
Limitation:
This CD40 antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P27512