CD41 Antibody / ITGA2B

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NSJ Bioreagents
Product Code: NSJ-RQ4069
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4069-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
After reconstitution, the CD41 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
FACS testing of human HL60 cells with CD41 antibody at 1ug/10^6 cells. CD41 antibody (blue), isotype control (green), cells alone (red).
2 / 7
Flow cytometry testing of human U937 cells with CD41 antibody at 1ug/10^6 cells. CD41 antibody (blue), isotype control (green), cells alone (red).
3 / 7
IHC testing of FFPE human rectal cancer tissue with CD41 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 7
IHC testing of FFPE human breast cancer tissue with CD41 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 7
Western blot testing of 1) human K562, 2) human Raji, 3) rat testis, 4) rat brain and 5) mouse brain lysate with CD41 antibody at 0.5ug/ml. Expected molecular weight ~120 kDa (alpha chain) and ~23 kDa (beta chain).
6 / 7
Immunofluorescent staining of FFPE human tonsil tissue with CD41 antibody at 1ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
7 / 7
Immunofluorescent staining of FFPE mouse lymph tissue with CD41 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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FACS testing of human HL60 cells with CD41 antibody at 1ug/10^6 cells. CD41 antibody (blue), isotype control (green), cells alone (red).
Flow cytometry testing of human U937 cells with CD41 antibody at 1ug/10^6 cells. CD41 antibody (blue), isotype control (green), cells alone (red).
IHC testing of FFPE human rectal cancer tissue with CD41 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human breast cancer tissue with CD41 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of 1) human K562, 2) human Raji, 3) rat testis, 4) rat brain and 5) mouse brain lysate with CD41 antibody at 0.5ug/ml. Expected molecular weight ~120 kDa (alpha chain) and ~23 kDa (beta chain).
Immunofluorescent staining of FFPE human tonsil tissue with CD41 antibody at 1ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE mouse lymph tissue with CD41 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml
Application Note:
Optimal dilution of the CD41 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Integrin alpha-IIb, also called CD41, is a protein that in humans is encoded by the ITGA2B gene. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human partial protein corresponding to amino acids L32-D263 was used as the immunogen for the CD41 antibody.
Limitation:
This CD41 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P08514