CD43 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31521
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31521-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD43 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human Jurkat cell lysate with CD43 antibody. Expected molecular weight: 45-115 kDa depending on glycosylation level.
2 / 10
IHC-P: CD43 antibody testing of human tonsil tissue. HIER: steamed with pH6 citrate buffer.
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IHC-P: CD43 antibody testing of mouse spleen tissue. HIER: steamed with pH6 citrate buffer.
4 / 10
IHC-P: CD43 antibody testing of rat spleen tissue. HIER: steamed with pH6 citrate buffer.
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ICC staining of FFPE human K562 cells with CD43 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
6 / 10
Flow cytometry testing of mouse P3NSI cells with CD43 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD43 antibody.
7 / 10
Flow cytometry testing of rat BRL 3A cells with CD43 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD43 antibody.
8 / 10
Flow cytometry testing of human HL60 cells with CD43 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD43 antibody.
9 / 10
Immunofluorescent staining of FFPE human tonsil tissue with CD43 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
10 / 10
Western blot testing of CD43 antibody (0.5ug/ml) and recombinant human protein (0.5ng)
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Western blot testing of human Jurkat cell lysate with CD43 antibody. Expected molecular weight: 45-115 kDa depending on glycosylation level.
IHC-P: CD43 antibody testing of human tonsil tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: CD43 antibody testing of mouse spleen tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: CD43 antibody testing of rat spleen tissue. HIER: steamed with pH6 citrate buffer.
ICC staining of FFPE human K562 cells with CD43 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of mouse P3NSI cells with CD43 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD43 antibody.
Flow cytometry testing of rat BRL 3A cells with CD43 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD43 antibody.
Flow cytometry testing of human HL60 cells with CD43 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD43 antibody.
Immunofluorescent staining of FFPE human tonsil tissue with CD43 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of CD43 antibody (0.5ug/ml) and recombinant human protein (0.5ng)

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CD43 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CD43, also known as leukosialin or sialophorin, is a transmembrane cell surface protein that in humans is encoded by the SPN gene. It is mapped to 16p11.2. It is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which is important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Expression of CD43 is deficient and/or defective in the X-chromosome-linked immunodeficiency disorder Wiscott-Aldrich syndrome, suggesting that CD43 have a role in T-cell activation. T-cell activation requires the removal of CD43 from the immunologic synapse to allow efficient engagement of the TCR with molecules on the APC.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
6693
Immunogen:
Human partial recombinant protein (AA 272-400) was used as the immunogen for this CD43 antibody.
Limitation:
This CD43 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat