CD44 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R31746
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31746-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD44 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human tonsil with CD44 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Immunofluorescent staining of FFPE rat lymph tissue with CD44 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 4
IHC staining of FFPE human intestinal cancer with CD44 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 4
Western blot testing of 1) human HeLa, 2) human U-87 MG, 3) rat PC-12 and 4) rat C6 cell lysate with CD44 antibody. Predicted molecular weight ~82 kDa, but may be observed at higher molecular weights due to glycosylation.
❮❯

Immunofluorescent staining of FFPE human tonsil with CD44 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat lymph tissue with CD44 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human intestinal cancer with CD44 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human U-87 MG, 3) rat PC-12 and 4) rat C6 cell lysate with CD44 antibody. Predicted molecular weight ~82 kDa, but may be observed at higher molecular weights due to glycosylation.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CD44 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cluster of differentiation 44 is also known as LHR or MC56. CD44 is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for its gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
960
Immunogen:
An amino acid sequence from the N-terminus of human CD44 (RFAGVFHVEKNGRYSISRTEAADLCKAF) was used as the immunogen for this CD44 antibody.
Limitation:
This CD44 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat