CD59 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31963
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31963-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
After reconstitution, the CD59 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human A431 cells with CD59 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Flow cytometry testing of human K562 cells with CD59 antibody at 1ug/10^6 cells; Red=cells alone, Green=isotype control, Blue=CD59 antibody.
3 / 4
IHC testing of FFPE human tonsil with CD59 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 4
Western blot testing of human 1) HeLa and 2) PC-3 cell lysate with CD59 antibody. Expected molecular weight: 14-20 kDa depending on level of glycosylation.
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Immunofluorescent staining of FFPE human A431 cells with CD59 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human K562 cells with CD59 antibody at 1ug/10^6 cells; Red=cells alone, Green=isotype control, Blue=CD59 antibody.
IHC testing of FFPE human tonsil with CD59 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Western blot testing of human 1) HeLa and 2) PC-3 cell lysate with CD59 antibody. Expected molecular weight: 14-20 kDa depending on level of glycosylation.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/10^6 cells,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the CD59 antibody should be determined by the researcher.
Description:
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. And this protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 26-102 of human CD59 were used as the immunogen for the CD59 antibody.
Limitation:
This CD59 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P13987