CD79a Antibody

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NSJ Bioreagents
Product Code: NSJ-R31585
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31585-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD79a antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human tonsil tissue with CD79a antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human tonsil tissue with CD79a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) Daudi and 2) Ramos cell lysate with CD79a antibody. Expected molecular weight: 25~47 kDa depending on glycosylation level.
4 / 4
Flow cytometry testing of human PBM cells with CD79a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD79a antibody.
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Immunofluorescent staining of FFPE human tonsil tissue with CD79a antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with CD79a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Daudi and 2) Ramos cell lysate with CD79a antibody. Expected molecular weight: 25~47 kDa depending on glycosylation level.
Flow cytometry testing of human PBM cells with CD79a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD79a antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CD79a antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cluster of differentiation 79A also known as B-cell antigen receptor complex-associated protein alpha chain and MB-1 membrane glycoprotein, is a protein that in humans is encoded by the CD79A gene. It is mapped to 19q13.2. CD79a is a membrane protein with an extracellular immunoglobulin domain, a single span transmembrane region and a short cytoplasmic domain. Genetic deletion of the transmembrane exon of CD79A results in loss of protein and a complete block of B cell development at the pro to pre B cell transition.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
973
Immunogen:
Human partial recombinant protein (AA 121-226) was used as the immunogen for this CD79a antibody.
Limitation:
This CD79a antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat