CD8 Antibody

NSJ Bioreagents
Product Code: NSJ-R31662
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31662-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD8 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of CD8 antibody and Lane 1: Jurkat; 2: Raji; 3: HL-60; 4: A549; 5: COLO320; 6: HeLa lysate; Predicted size: 34KD; Observed size: 34~60KD depending on glycosylation level
2 / 3
Western blot testing of CD8 antibody and recombinant human protein (0.5ng)
3 / 3
IHC-P: CD8 antibody testing of human tonsil tissue

Western blot testing of CD8 antibody and Lane 1: Jurkat; 2: Raji; 3: HL-60; 4: A549; 5: COLO320; 6: HeLa lysate; Predicted size: 34KD; Observed size: 34~60KD depending on glycosylation level
Western blot testing of CD8 antibody and recombinant human protein (0.5ng)
IHC-P: CD8 antibody testing of human tonsil tissue

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CD8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CD8 is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. CD8, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
925
Immunogen:
Human partial recombinant protein (AA 22-187) was used as the immunogen for this CD8 antibody. This amino acid sequence is from the alpha subunit.
Limitation:
This CD8 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human