CD81 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4082
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4082-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
After reconstitution, the CD81 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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FACS testing of human PBMCs with CD81 antibody at 1ug/10^6 cells. Blue=CD81 antibody, Green=isotype control, Red=cells alone.
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FACS testing of human Jurkat cells with CD81 antibody at 1ug/10^6 cells. Blue=CD81 antibody, Green=isotype control, Red=cells alone.
3 / 4
FACS testing of human K562 cells with CD81 antibody at 1ug/10^6 cells. Blue=CD81 antibody, Green=isotype control, Red=cells alone.
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IHC testing of FFPE human tonsil tissue with CD81 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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FACS testing of human PBMCs with CD81 antibody at 1ug/10^6 cells. Blue=CD81 antibody, Green=isotype control, Red=cells alone.
FACS testing of human Jurkat cells with CD81 antibody at 1ug/10^6 cells. Blue=CD81 antibody, Green=isotype control, Red=cells alone.
FACS testing of human K562 cells with CD81 antibody at 1ug/10^6 cells. Blue=CD81 antibody, Green=isotype control, Red=cells alone.
IHC testing of FFPE human tonsil tissue with CD81 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CD81 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CD81, also known as TAPA1, is a protein which in humans is encoded by the CD81 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human partial protein corresponding to amino acids F113-K201 was used as the immunogen for the CD81 antibody.
Limitation:
This CD81 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P60033