Cd81 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4083
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4083-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the Cd81 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE mouse spleen with Cd81 antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE mouse liver with Cd81 antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing. Green=isotype control, Blue= Cd81 antibody.
3 / 6
Immunofluorescent staining of FFPE mouse RM-1 cells with Cd81 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 6
Flow cytometry testing of mouse spleen cells with Cd81 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd81 antibody.
5 / 6
Flow cytometry testing of mouse Neuro-2a cells with Cd81 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd81 antibody.
6 / 6
Western blot testing of mouse 1) thymus, 2) SP2/0 and 3) NIH 3T3 lysate with Cd81 antibody. Predicted molecular weight: 22-26 kDa.
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IHC staining of FFPE mouse spleen with Cd81 antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver with Cd81 antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing. Green=isotype control, Blue= Cd81 antibody.
Immunofluorescent staining of FFPE mouse RM-1 cells with Cd81 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of mouse spleen cells with Cd81 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd81 antibody.
Flow cytometry testing of mouse Neuro-2a cells with Cd81 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd81 antibody.
Western blot testing of mouse 1) thymus, 2) SP2/0 and 3) NIH 3T3 lysate with Cd81 antibody. Predicted molecular weight: 22-26 kDa.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Cd81 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CD81 molecule, also known as TAPA1, is a protein which in humans is encoded by the CD81 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse partial protein corresponding to amino acids K116-K201 was used as the immunogen for the Cd81 antibody.
Limitation:
This Cd81 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Mouse
Uniprot #:
P35762