CD82 Antibody

NSJ Bioreagents
Product Code: NSJ-R31587
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31587-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD82 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE human tonsil tissue with CD82 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human tonsil tissue with CD82 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of human 1) Jurkat, 2) Raji and 3) U-87 MG cell lysate with CD82 antibody. Expected molecular weight: 30-60 kDa depending on glycosylation level.

IHC staining of FFPE human tonsil tissue with CD82 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with CD82 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Jurkat, 2) Raji and 3) U-87 MG cell lysate with CD82 antibody. Expected molecular weight: 30-60 kDa depending on glycosylation level.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CD82 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cluster of Differentiation 82, also called KAI1, is a protein that in humans encoded by the CD82 gene. The gene is mapped to 11p11.2. This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. The expression of CD82 appears to be correlated with lymph node metastasis in esophageal squamous cell carcinoma (ESCC). And overexpression can suppress tumor invasiveness and metastatic potential by inducing MMP9 inactivation via upregulation of TIMP1.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
3732
Immunogen:
Human partial recombinant protein (AA 98-267) was used as the immunogen for this CD82 antibody.
Limitation:
This CD82 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human