CD86 Antibody

NSJ Bioreagents
Product Code: NSJ-R31664
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31664-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
The lyophilized CD86 antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freeze/thaws.

Images

1 / 3
Western blot testing of mouse 1) spleen, 2) thymus, 3) J774A.1 and 4) ANA-1 cell lysate with CD86 antibody. Predicted molecular weight ~38 kDa (unmodified), 45-70 kDa (glycosylated).
2 / 3
Western blot testing of human 1) Raji and 2) Daudi cell lysate with CD86 antibody. Predicted molecular weight ~38 kDa (unmodified), 45-70 kDa (glycosylated).
3 / 3
IHC staining of FFPE human tonsil tissue with CD86 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of mouse 1) spleen, 2) thymus, 3) J774A.1 and 4) ANA-1 cell lysate with CD86 antibody. Predicted molecular weight ~38 kDa (unmodified), 45-70 kDa (glycosylated).
Western blot testing of human 1) Raji and 2) Daudi cell lysate with CD86 antibody. Predicted molecular weight ~38 kDa (unmodified), 45-70 kDa (glycosylated).
IHC staining of FFPE human tonsil tissue with CD86 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Titration of the CD86 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cluster of Differentiation 86 (also known as B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. The antigen presentation coactivators B7-1 and B7-2/CD86, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
942
Immunogen:
Human partial recombinant protein (AA 24-329) was used as the immunogen for this CD86 antibody.
Limitation:
This CD86 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human