CD90 Antibody / Thy1

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NSJ Bioreagents
Product Code: NSJ-R32658
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32658-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD90 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
IHC testing of FFPE mouse spleen tissue with CD90 antibody. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to testing.
2 / 4
Immunofluorescent staining of FFPE rat brain tissue with CD90 antibody (green) and DAPI nuclear stain (blue). Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to testing.
3 / 4
Western blot testing of 1) mouse thymus, 2) mouse brain, 3) rat thymus and 4) rat brain tissue lysate with CD90 antibody. Predicted molecular weight 18~35 kDa depending on glycosylation level.
4 / 4
Flow cytometry testing of mouse HEPA1-6 cells with CD90 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD90 antibody.
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IHC testing of FFPE mouse spleen tissue with CD90 antibody. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to testing.
Immunofluorescent staining of FFPE rat brain tissue with CD90 antibody (green) and DAPI nuclear stain (blue). Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to testing.
Western blot testing of 1) mouse thymus, 2) mouse brain, 3) rat thymus and 4) rat brain tissue lysate with CD90 antibody. Predicted molecular weight 18~35 kDa depending on glycosylation level.
Flow cytometry testing of mouse HEPA1-6 cells with CD90 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD90 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the CD90 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
CD90 (Cluster of Differentiation 90) or Thy-1 is a heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. The CD90 gene is mapped to 11q23.3. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids Q20-C131 from the mouse protein were used as the immunogen for the CD90 antibody.
Limitation:
This CD90 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
P01831