CD95 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30285
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30285-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD95 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of rat 1) liver, 2) spleen, 3) brain and 4) heart lysate with CD95 antibody at 1ug/ml. Predicted molecular weight: 35-38 kDa (unmodified), 40-55 kDa (glycosylated).
2 / 4
Western blot testing of mouse 1) liver, 2) spleen, 3) brain, 4) kidney, 5) thymus, 6) lung, 7) Hepa 1-6 and 8) NIH 3T3 lysate with CD95 antibody. Predicted molecular weight: 35-38 kDa (unmodified), 40-55 kDa (glycosylated).
3 / 4
IHC-P: CD95 antibody testing of rat spleen tissue lysate. Required HIER: boil the paraffin sections in 10mM citrate buffer, pH6, for 20 mins.
4 / 4
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Western blot testing of rat 1) liver, 2) spleen, 3) brain and 4) heart lysate with CD95 antibody at 1ug/ml. Predicted molecular weight: 35-38 kDa (unmodified), 40-55 kDa (glycosylated).
Western blot testing of mouse 1) liver, 2) spleen, 3) brain, 4) kidney, 5) thymus, 6) lung, 7) Hepa 1-6 and 8) NIH 3T3 lysate with CD95 antibody. Predicted molecular weight: 35-38 kDa (unmodified), 40-55 kDa (glycosylated).
IHC-P: CD95 antibody testing of rat spleen tissue lysate. Required HIER: boil the paraffin sections in 10mM citrate buffer, pH6, for 20 mins.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CD95 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
FAS(also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors, can induce apoptosis or, conversely, can deliver growth stimulatory signals. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor(TNF) receptors and the low-affinity nerve growth factor receptor(NGFR) and is mapped to 10q24.1. And the FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of rat CD95 (YTDRKHYSDKCRRCAFCDEGHGL) was used as the immunogen for this CD95 antibody.
Limitation:
This CD95 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
Q63199