Cdc25B Antibody

Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the Cdc25B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 humanCDC25genes code for the A, B, and C forms of CDC25.CDC25Bis mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, andCDC25Cat serine-216; phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Amino acids 541-559 (DYRPMNHEAFKDELKTFRL-human) were used as the immunogen for this Cdc25B antibody.

This Cdc25B antibody is available for research use only.

Antigen affinity

Human, Rat

P30305