CDC45 Antibody / PORC-PI-1

NSJ Bioreagents
Product Code: NSJ-RQ4295
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4295-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the CDC45 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of rat 1) testis, 2) thymus, 3) brain, 4) lung and mouse 5) testis, 6) thymus, 7) brain, 8) lung and 9) HEPA1-6 lysate with CDC45 antibody at 0.5ug/ml. Predicted molecular weight ~66 kDa.

Western blot testing of rat 1) testis, 2) thymus, 3) brain, 4) lung and mouse 5) testis, 6) thymus, 7) brain, 8) lung and 9) HEPA1-6 lysate with CDC45 antibody at 0.5ug/ml. Predicted molecular weight ~66 kDa.

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CDC45 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Cell division control protein 45 homolog was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids E166-A431 was used as the immunogen for the CDC45 antibody.
Limitation:
This CDC45 antibody is available for research use only.
Localization:
Cytoplasm, nucleus
Purity:
Antigen affinity purified
Species Reactivity :
Mouse, Rat
Species Reactivity (Predicted):
Human
Uniprot #:
O75419