Chk2 Antibody

Western blot: 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the Chk2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

CHK2, a protein kinase that is activated in response to DNA damage, is involved in cell cycle arrest. Mapped on 22q12.1, CHK2 has a potential regulatory region rich in SQ and TQ amino acid pairs. It regulates BRCA1 function after DNA damage by phosphorylating serine-988 of BRCA1. Additionally, CHK2 can be modified by phosphorylation and activated in response to ionizing radiation, and can be also modified in response to hydroxyurea treatment. Furthermore, oligomerization of CHEK2 increases the efficiency of transautophosphorylation, resulting in the release of active CHEK2 monomers that proceed to enforce checkpoint control in irradiated cells. Morever, CHK2 is a tumor suppressor gene conferring predisposition to sarcoma, breast cancer, and brain tumors, and that their observations provided a link between the central role of p53 inactivation in human cancer and the well-defined G2 checkpoint in yeast. There is a wide expression of small amounts of CHK2 mRNA with larger amounts in human testis, spleen, colon, and peripheral blood leukocytes.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

An amino acid sequence from the C-terminus of human Chk2 (FSEHRTQVSLKDQIT) was used as the immunogen for this Chk2 antibody.

This Chk2 antibody is available for research use only.

Antigen affinity

Human, Mouse, Rat

O96017