c-Kit Antibody

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NSJ Bioreagents
Product Code: NSJ-R31671
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31671-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the c-Kit antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of c-Kit antibody and HEPG2 lysate
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Western blot testing of c-Kit antibody and recombinant human protein (0.5ng)
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IHC-P: c-Kit antibody testing of human intestinal cancer tissue
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Flow cytometry testing of human K562 cells with c-Kit antibody at 1ug/10^6 cells (cells blocked with goat sera); Red=cells alone, Green=isotype control, Blue=c-Kit antibody.
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Western blot testing of c-Kit antibody and HEPG2 lysate
Western blot testing of c-Kit antibody and recombinant human protein (0.5ng)
IHC-P: c-Kit antibody testing of human intestinal cancer tissue
Flow cytometry testing of human K562 cells with c-Kit antibody at 1ug/10^6 cells (cells blocked with goat sera); Red=cells alone, Green=isotype control, Blue=c-Kit antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the c-Kit antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SCFR (Mast/stem cell growth factor receptor), also known as CD117, is a protein that in humans is encoded by the KIT gene. c-Kit was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. It is expressed on the surface of germ cells up to the pachytene stage. Signaling from the receptor, tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the c-Kit effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
3815
Immunogen:
Human partial recombinant protein (AA 26-285) was used as the immunogen for this c-Kit antibody. Human protein shares 66% amino acid sequence identity with mouse protein.
Limitation:
This c-Kit antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human