CLPP Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4278
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4278-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CLPP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 11
Immunofluorescent staining of FFPE human MCF7 cells with CLPP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 11
IHC testing of FFPE human ovarian cancer tissue with CLPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 11
IHC testing of FFPE human gallbladder adenocarcinoma tissue with CLPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 11
IHC testing of FFPE human breast cancer tissue with CLPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC testing of FFPE human lung cancer tissue with CLPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC testing of FFPE human liver cancer tissue with CLPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC testing of FFPE human lung cancer tissue with CLPP antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
8 / 11
Western blot testing of human 1) PC-3, 2) HEK293, 3) MCF7, 4) ThP-1, 5) rat pancreas, 6) rat lung and 7) mouse pancreas lysate with CLPP antibody at 0.5ug/ml. Predicted molecular weight: 26-30 kDa.
9 / 11
Western blot testing of human A375 cell lysate with CLPP antibody. Predicted molecular weight: 26-30 kDa.
10 / 11
Flow cytometry testing of human U937 cells with CLPP antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CLPP antibody.
11 / 11
Flow cytometry testing of human A431 cells with CLPP antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CLPP antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with CLPP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human ovarian cancer tissue with CLPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC testing of FFPE human gallbladder adenocarcinoma tissue with CLPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC testing of FFPE human breast cancer tissue with CLPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC testing of FFPE human lung cancer tissue with CLPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC testing of FFPE human liver cancer tissue with CLPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC testing of FFPE human lung cancer tissue with CLPP antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) PC-3, 2) HEK293, 3) MCF7, 4) ThP-1, 5) rat pancreas, 6) rat lung and 7) mouse pancreas lysate with CLPP antibody at 0.5ug/ml. Predicted molecular weight: 26-30 kDa.
Western blot testing of human A375 cell lysate with CLPP antibody. Predicted molecular weight: 26-30 kDa.
Flow cytometry testing of human U937 cells with CLPP antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CLPP antibody.
Flow cytometry testing of human A431 cells with CLPP antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CLPP antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CLPP antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
ATP-dependent Clp protease proteolytic subunit (ClpP) is an enzyme that in humans is encoded by the CLPP gene. It is mapped to 19p13.3. The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids A184-H247 was used as the immunogen for the CLPP antibody.
Limitation:
This CLPP antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q16740