Collagen I Antibody / COL1A1

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NSJ Bioreagents
Product Code: NSJ-R31258
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31258-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution the Collagen type 1 antibody can be stored for up to one month at 4 C. For long-term aliquot and store at -20 C. Avoid repeated freezing and thawing.

Images

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Western blot testing of Collagen type 1 antibody and Lane 1: rat lung; 2: human placenta; 3: rat testis tissue lysate. Expected molecular weight: 140-210 kDa (precusor), 70-90 kDa (mature).
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IHC-P: Collagen 1 antibody testing of human intestine cancer tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC-F testing of Collagen type 1 antibody and human placenta tissue
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ICC testing of Collagen 1 antibody and NIH3T3 cells. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC-P: Collagen 1 antibody testing of rat lung tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human breast tissue with Collagen 1 antibody (red) and DAPI counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human tonsil tissue with Collagen 1 antibody (red) and DAPI counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
8 / 10
IHC-P: Collagen 1 antibody testing of mouse lung tissue at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
9 / 10
IHC-P: Collagen 1 antibody testing of mouse lung tissue at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
10 / 10
IHC-P: Collagen 1 antibody testing of rat lung tissue at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of Collagen type 1 antibody and Lane 1: rat lung; 2: human placenta; 3: rat testis tissue lysate. Expected molecular weight: 140-210 kDa (precusor), 70-90 kDa (mature).
IHC-P: Collagen 1 antibody testing of human intestine cancer tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC-F testing of Collagen type 1 antibody and human placenta tissue
ICC testing of Collagen 1 antibody and NIH3T3 cells. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC-P: Collagen 1 antibody testing of rat lung tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human breast tissue with Collagen 1 antibody (red) and DAPI counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human tonsil tissue with Collagen 1 antibody (red) and DAPI counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC-P: Collagen 1 antibody testing of mouse lung tissue at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC-P: Collagen 1 antibody testing of mouse lung tissue at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC-P: Collagen 1 antibody testing of rat lung tissue at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Collagen type 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Collagen type 1 alpha 1, also known as COL1A1, is a human gene that encodes the major component of type 1 collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type 1 collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Collagen type 1 is fibril-forming and found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of mouse COL1A1 (QPPQEKSQDGGRYYRA) was used as the immunogen for this Collagen type 1 antibody (100% rat homology).
Limitation:
This Collagen type 1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P02452