Collagen III Antibody / COL3A1

NSJ Bioreagents
Product Code: NSJ-R30026
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30026-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: Col-29, aka FH-7A
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Western Blot (WB)
Storage:
After reconstitution, the COL3A1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of COL3A1 antibody and Lane 1: HT1080; 2: COLO320 cell lysate. Expected molecular weight ~139 kDa, observed here at ~70 kDa.~
2 / 2
IHC testing of frozen mouse kidney tissue with COL3A1 antibody.

Western blot testing of COL3A1 antibody and Lane 1: HT1080; 2: COLO320 cell lysate. Expected molecular weight ~139 kDa, observed here at ~70 kDa.~
IHC testing of frozen mouse kidney tissue with COL3A1 antibody.

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml-formalin/acetone fixed tissues
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the COL3A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Collagen type III, also known as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
1281
Immunogen:
Human collagen type III was used as the immunogen for this COL3A1 antibody.
Limitation:
This COL3A1 antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, rat