Collagen IV Antibody / COL4A2

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NSJ Bioreagents
Product Code: NSJ-R30596
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30596-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The lyophilized Collagen IV antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC-F testing of Canstatin / Collagen IV antibody and rat kidney tissue
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IHC testing of FFPE mouse lung tissue with Canstatin / Collagen IV antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of rat 1) kidney and 2) lung lysate with Canstatin / Collagen IV antibody at 0.5ug/ml. Expected molecular weight of Canstatin: ~24 kDa.
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IHC-F testing of Canstatin / Collagen IV antibody and rat kidney tissue
IHC testing of FFPE mouse lung tissue with Canstatin / Collagen IV antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of rat 1) kidney and 2) lung lysate with Canstatin / Collagen IV antibody at 0.5ug/ml. Expected molecular weight of Canstatin: ~24 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
Titration of the Collagen IV antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, is one of the six subunits of Collagen IV and possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Collagen IV COL4A2 (amino acids 1697-1712: LIRTHISRCQVCMKNL) was used as the immunogen for this Collagen IV antibody (100% homologous in human, mouse and rat). This amino acid sequence is from the Canstatin fragment of the protein.
Limitation:
This Collagen IV antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P08572