Complement Factor I Antibody / CFI

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NSJ Bioreagents
Product Code: NSJ-R32399
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32399-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Factor I antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of 1) rat liver and 2) human HeLa lysate with Factor I antibody. Expected molecular weight: 66 kDa (unmodified), 88 kDa (fully glycosylated), 50/38 kDa (fully glycosylated heavy/light chain).
2 / 3
Flow cytometry testing of human HepG2 cells with Factor I antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Factor I antibody.
3 / 3
Flow cytometry testing of human U-87 MG cells with Factor I antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Factor I antibody.
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Western blot testing of 1) rat liver and 2) human HeLa lysate with Factor I antibody. Expected molecular weight: 66 kDa (unmodified), 88 kDa (fully glycosylated), 50/38 kDa (fully glycosylated heavy/light chain).
Flow cytometry testing of human HepG2 cells with Factor I antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Factor I antibody.
Flow cytometry testing of human U-87 MG cells with Factor I antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Factor I antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Flow cytometry: 1-3ug/million cells
Description:
Complement factor I, also known as C3b/C4b inactivator, is a protein that in humans is encoded by the CFI gene. This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids K19-D220 were used as the immunogen for the Factor I antibody.
Limitation:
This Factor I antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
P05156