COMT Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R31326
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31326-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the COMT antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of human 1) HeLa, 2) A375 and 3) PANC cell lysate with COMT antibody. Predicted molecular weight: ~30/25kDa (isoforms 1/2).
2 / 4
IHC-P: COMT antibody testing of human lung cancer tissue
3 / 4
IHC-P: COMT antibody testing of human kidney cancer tissue
4 / 4
Western blot testing of 1) human placenta, 2) human K562, 3) human SH-SY5Y, 4) rat brain, 5) rat liver, 6) mouse brain and 7) mouse liver lysate with COMT antibody. Predicted molecular weight: ~30/25 kDa (isoforms 1/2).
❮❯

Western blot testing of human 1) HeLa, 2) A375 and 3) PANC cell lysate with COMT antibody. Predicted molecular weight: ~30/25kDa (isoforms 1/2).
IHC-P: COMT antibody testing of human lung cancer tissue
IHC-P: COMT antibody testing of human kidney cancer tissue
Western blot testing of 1) human placenta, 2) human K562, 3) human SH-SY5Y, 4) rat brain, 5) rat liver, 6) mouse brain and 7) mouse liver lysate with COMT antibody. Predicted molecular weight: ~30/25 kDa (isoforms 1/2).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the COMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Catechol O-methyltransferase, also called COMT, is one of the major mammalian enzymes involved in the metabolic degradation of catecholamines. This gene is mapped to 22q11.21. Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. It is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between the two forms reside within the N-termini.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human Catechol O-methyltransferase (DKKGKIVDAVIQEHQ) was used as the immunogen for this COMT antibody.
Limitation:
This COMT antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P21964