Connexin 43 Antibody / Cx43

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NSJ Bioreagents
Product Code: NSJ-R30200
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30200-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Connexin 43 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing 1) human placenta, 2) rat brain, 3) rat heart, 4) mouse brain and 5) mouse heart lysate with Connexin 43 antibody at 0.5ug/ml. Predicted molecular weight: ~43 kDa.
2 / 3
Immunofluorescent staining of FFPE rat heart tissue with Connexin 43 antibody (green) at 1ug/ml and DAPI nuclear stain. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
IHC staining of FFPE rat heart tissue with Connexin 43 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing 1) human placenta, 2) rat brain, 3) rat heart, 4) mouse brain and 5) mouse heart lysate with Connexin 43 antibody at 0.5ug/ml. Predicted molecular weight: ~43 kDa.
Immunofluorescent staining of FFPE rat heart tissue with Connexin 43 antibody (green) at 1ug/ml and DAPI nuclear stain. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat heart tissue with Connexin 43 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Connexin 43 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Connexin 43 (Cx43), also called GAP Junction Protein, alpha-1(GJA1), is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin 43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 also plays a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Connexin 43 (RASSRASSRPRPDDLEI) was used as the immunogen for this Connexin 43 antibody (100% conserved in mouse and rat).
Limitation:
This Connexin 43 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P17302