Connexin 43 Antibody / Cx43

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NSJ Bioreagents
Product Code: NSJ-R30201
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30201-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The lyophilized Cx43 antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC-P: Cx43 antibody testing of human lung cancer tissue
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IHC-P: Cx43 antibody testing of rat heart tissue
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IHC-F testing of rat heart tissue
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Western blot testing of 1) human placenta, 2) rat brain, 3) rat heart, 4) mouse brain and 5) mouse heart lysate with Cx43 antibody at 0.5ug/ml. Predicted molecular weight ~43 kDa.
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IHC testing of human intestinal cancer tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of human breast cancer tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of mouse heart tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of rat heart tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC-P: Cx43 antibody testing of human lung cancer tissue
IHC-P: Cx43 antibody testing of rat heart tissue
IHC-F testing of rat heart tissue
Western blot testing of 1) human placenta, 2) rat brain, 3) rat heart, 4) mouse brain and 5) mouse heart lysate with Cx43 antibody at 0.5ug/ml. Predicted molecular weight ~43 kDa.
IHC testing of human intestinal cancer tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of human breast cancer tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of mouse heart tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of rat heart tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
Titration of the Cx43 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Connexin 43 (Cx43), also called GAP Junction Protein, alpha, is a member of the connexin gene family and is abundantly expressed in the heart and liver. Connexin 43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Cx43 also plays a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 351-367 (HELQPLAIVDQRPSSRA-human) were used as the immunogen for this Cx43 antibody (100% conserved in mouse and rat).
Limitation:
This Cx43 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P17302