COPE Antibody / Coatomer subunit epsilon

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NSJ Bioreagents
Product Code: NSJ-R32959
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32959-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the COPE antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat stomach, 2) rat small intestine, 3) rat pancreas, 4) mouse stomach, 5) mouse pancreas, 6) human MCF7, 7) human HeLa and 8) human 22RV1 lysate with COPE antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa.
2 / 6
IHC testing of FFPE human breast cancer tissue with COPE antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 6
IHC testing of FFPE mouse pancreas tissue with COPE antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 6
IHC testing of FFPE rat pancreas tissue with COPE antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 6
IF/ICC staining of FFPE human U-2 OS cells with COPE antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
6 / 6
Flow cytometry testing of human HepG2 cells with COPE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= COPE antibody.
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Western blot testing of 1) rat stomach, 2) rat small intestine, 3) rat pancreas, 4) mouse stomach, 5) mouse pancreas, 6) human MCF7, 7) human HeLa and 8) human 22RV1 lysate with COPE antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa.
IHC testing of FFPE human breast cancer tissue with COPE antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse pancreas tissue with COPE antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat pancreas tissue with COPE antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IF/ICC staining of FFPE human U-2 OS cells with COPE antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HepG2 cells with COPE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= COPE antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence/Immunocytochemistry (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the COPE antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Coatomer subunit epsilon is a protein that in humans is encoded by the COPE gene. The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids E80-A308 was used as the immunogen for the COPE antibody.
Limitation:
This COPE antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O14579