COX2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30815
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30815-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The lyophilized COX2 antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC testing of FFPE differentiated adenocarcinoma in human appendix tissue with COX-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC testing of FFPE human liver cancer tissue with COX-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC testing of FFPE human lung adenocarcinoma tissue with COX-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC testing of FFPE human colonic adenocarcinoma tissue with COX-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
IHC testing of FFPE mouse lung tissue with COX-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 6
Western blot testing of 1) human HeLa, 2) mouse thymus and 3) LPS-stimulated mouse RAW264.7 cell lysate with COX-2 antibody. Predicted molecular weight ~69 kDa.
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IHC testing of FFPE differentiated adenocarcinoma in human appendix tissue with COX-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC testing of FFPE human liver cancer tissue with COX-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC testing of FFPE human lung adenocarcinoma tissue with COX-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC testing of FFPE human colonic adenocarcinoma tissue with COX-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC testing of FFPE mouse lung tissue with COX-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) mouse thymus and 3) LPS-stimulated mouse RAW264.7 cell lysate with COX-2 antibody. Predicted molecular weight ~69 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
Titration of the COX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cyclooxygenase (COX) is the key enzyme in conversion of arachidonic acid to PGs. The COX2 gene encodes an inducible prostaglandin synthase enzyme that is overexpressed in adenocarcinomas and other tumors. Deletion of the murine gene in Min mice reduced the incidence of intestinal tumors, suggesting that it is required for tumorigenesis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 589-604 (DDINPTVLLKERSTEL-human) were used as the immunogen for this COX2 antibody.
Limitation:
This COX2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P35354