CrkL Antibody

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NSJ Bioreagents
Product Code: NSJ-R30906
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30906-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the CrkL antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of fixed and permeabilized human U-251 cells with CrkL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CrkL antibody.~
2 / 2
Western blot testing of 1) human K562, 2) human 293
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Flow cytometry testing of fixed and permeabilized human U-251 cells with CrkL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CrkL antibody.~
Western blot testing of 1) human K562, 2) human 293

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CrkL antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
v-Crk sarcoma virus CT10 oncogene homolog(avian)-like), also called CrkL, encodes a 303-amino acid polypeptide with a predicted molecular mass of ~36 kD. While CrkL is not the human homolog of CRK, it is similar to protein-tyrosine kinases with SH2 and SH3 (src homology) domains. By fluorescence in situ hybridization,the gene is localized to chromosome 22q11, centromeric of the chronic myelogenous leukemia breakpoint region. Senechal et al.(1996)showed that CRKL becomes phosphorylated when overexpressed, activates Ras-dependent and JNK pathways, and transforms fibroblasts. The authors also found CrkL to be a substrate for the BCR-ABL tyrosine kinase, leading them to conclude that it is a tyrosine kinase and an oncogene. CrkLwas required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 286-303 (FPFTHVKIFDPQNPDENE-human) were used as the immunogen for this CrkL antibody.
Limitation:
This CrkL antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P46109