CTBP2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32151
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32151-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CTBP2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) COLO320, 2) 293, and 3) HeLa lysate with CTBP2 antibody. Predicted/observed molecular weight ~49 kDa.
2 / 8
IHC testing of FFPE human intestinal cancer with CTBP2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 8
IHC testing of FFPE mouse skeletal muscle with CTBP2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 8
IHC testing of FFPE rat skeletal muscle with CTBP2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
5 / 8
Immunofluorescent staining of FFPE human colon with CTBP2 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
6 / 8
Immunofluorescent staining of FFPE human U-2 OS cells with CTBP2 antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
7 / 8
Immunofluorescent staining of FFPE mouse intestine with CTBP2 antibody (green) at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
8 / 8
Flow cytometry testing of human SiHa cells with CTBP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CTBP2 antibody.
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Western blot testing of human 1) COLO320, 2) 293, and 3) HeLa lysate with CTBP2 antibody. Predicted/observed molecular weight ~49 kDa.
IHC testing of FFPE human intestinal cancer with CTBP2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse skeletal muscle with CTBP2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat skeletal muscle with CTBP2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Immunofluorescent staining of FFPE human colon with CTBP2 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with CTBP2 antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE mouse intestine with CTBP2 antibody (green) at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human SiHa cells with CTBP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CTBP2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (Paraffin): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence (FFPE): 1-3ug/ml
Application Note:
Optimal dilution of the CTBP2 antibody should be determined by the researcher.
Description:
The E1a region of group C adenoviruses encodes 2 nearly identical proteins that are largely responsible for the oncogenic properties of adenoviruses. The CTBP1 protein binds to the C-terminal half of these E1A proteins. It's predicted that CTBP2 is a 445-amino acid protein and it is 72% identical to CTBP1. The CTBP2 gene is mapped to chromosome 10q26.13. CTBP2 is a mammalian corepressor that targets diverse transcriptional regulators. It bounds the short medial portion of delta-EF1 containing the PLDLSL motif and it enhances transrepression activity of delta-EF1.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 321-445 of human CTBP2 were used as the immunogen for the CTBP2 antibody.
Limitation:
This CTBP2 antibody is available for research use only.
Localization:
Nuclear, cell junctions
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P56545