CUEDC2 Antibody
| Code | Size | Price |
|---|
| NSJ-R31066-100ug | 100 ug | £535.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
After reconstitution, the CUEDC2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
Documents
Further Information
Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CUEDC2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cue Domain-Containing Protein 2 is involved in ubiquitin-and proteasome-mediated degradation of progesterone receptor (PR, or PGR) and estrogen receptor(ER)-alpha. Hartz(2011) mapped the CUEDC2 gene to chromosome 10q24.32 based on an alignment of the sequence with the genomic sequence (GRCh37). Using coimmunoprecipitation analysis and protein pull-down assays, Zhang et al.(2007) confirmed that CUEDC2 interacted with the PRB isoform of PR. Mutation analysis revealed that the IF domain of PRB and the CUE domain of CUEDC2 were required for the interaction. CUEDC2 reduced PRB protein content and promoted progesterone-induced PRB degradation via the ubiquitin-proteasome pathway.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Cue Domain-Containing Protein 2 (EAEEMKATYINLKPARKYRFH) was used as the immunogen for this CUEDC2 antibody (100% mouse homology).
Limitation:
This CUEDC2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9H467