CUL3 Antibody (Cullin-3)

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NSJ Bioreagents
Product Code: NSJ-R31039
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31039-100ug100 ug£535.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the CUL3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human HeLa, 2) K562, 3) Ramos, 4) rat brain, 5) mouse brain and 6) mouse NIH 3T3 cell lysate with CUL3 antibody. Expected molecular weight: ~89 kDa.
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Western blot testing of 1) human HeLa, 2) K562, 3) Ramos, 4) rat brain, 5) mouse brain and 6) mouse NIH 3T3 cell lysate with CUL3 antibody. Expected molecular weight: ~89 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CUL3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cullin-3 is a protein that in humans is encoded by the CUL3 gene. Kipreos et al.(1996) identified a conserved gene family, designated cullins, with at least 5 members in nematodes, 6 in humans, and 3 in S. cerevisiae. Hartz (2012) mapped the gene to chromosome 2q36.2 based on an alignment of the CUL3 sequence with the genomic sequence (GRCh37). Sumara et al.(2007) found that KLHL9, KLHL13, and CUL-3 interacted directly in a 370-kD protein complex in HeLa cell lysates. Rondou et al.(2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex directed ubiquitination of dopamine receptor D4(DRD4). KLHL12 interacted directly with CUL-3 and with the polymorphic intracellular loop-3 of D4. By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Kigoshi et al.(2011) showed that KLHL7 interacted with CUL-3 and ROC1(RBX1).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human Cullin-3 (DVFERYYKQHLARRLLTNK) was used as the immunogen for this CUL3 antibody (100% homologous in human, mouse and rat).
Limitation:
This CUL3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13618