Cyclin A2 Antibody / CCNA2

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NSJ Bioreagents
Product Code: NSJ-R31999
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31999-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cyclin A2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human U-2 OS cells with Cyclin A2 antibody at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
Immunofluorescent staining of FFPE human A431 cells with Cyclin A2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 5
IHC staining of FFPE human colorectal adenocarcinoma tissue with Cyclin A2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) human K562, 2) human HL60, 3) human MCF7, 4) rat stomach, 5) rat testis and 6) mouse stomach tissue lysate with Cyclin A2 antibody. Expected molecular weight ~49 kDa.
5 / 5
Flow cytometry testing of human U-2 OS cells with Cyclin A2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cyclin A2 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Cyclin A2 antibody at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human A431 cells with Cyclin A2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human colorectal adenocarcinoma tissue with Cyclin A2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human K562, 2) human HL60, 3) human MCF7, 4) rat stomach, 5) rat testis and 6) mouse stomach tissue lysate with Cyclin A2 antibody. Expected molecular weight ~49 kDa.
Flow cytometry testing of human U-2 OS cells with Cyclin A2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cyclin A2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence/Immunocytochemistry (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Cyclin A2 antibody should be determined by the researcher.
Description:
Cyclin A2, known as CCNA2, is mapped to 4q27. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. And Cyclin A2 is synthesized at the onset of S phase and localizes to the nucleus, where the cyclin A2-CDK2 complex is implicated in the initiation and progression of DNA synthesis. Phosphorylation of CDC6 and MCM4 by the cyclin A2-CDK2 complex prevents re-replication of DNA during the cell cycle.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 10-168 of human Cyclin A2 were used as the immunogen for the Cyclin A2 antibody.
Limitation:
This Cyclin A2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
P20248