Cyclin B1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31529
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31529-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Cyclin B1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Cyclin B1 antibody and Lane 1: HeLa; 2: 293T; 3: MCF-7; 4: COLO320 lysate. Predicted molecular weight: 48-60 kDa
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Western blot testing of Cyclin B1 antibody and recombinant human protein (0.5ng)
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Flow cytometry testing of human THP-1 cells with Cyclin B1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Cyclin B1 antibody.
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Immunofluorescent staining of FFPE human A431 cells with Cyclin B1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of Cyclin B1 antibody and Lane 1: HeLa; 2: 293T; 3: MCF-7; 4: COLO320 lysate. Predicted molecular weight: 48-60 kDa
Western blot testing of Cyclin B1 antibody and recombinant human protein (0.5ng)
Flow cytometry testing of human THP-1 cells with Cyclin B1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Cyclin B1 antibody.
Immunofluorescent staining of FFPE human A431 cells with Cyclin B1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Cyclin B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cyclin B1 is a protein that in humans is encoded by the CCNB1 gene, and it is mapped to 5q13.2. It is a regulatory protein involved in mitosis. The gene product complexes withp34(Cdc2)to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. CCNB1 contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once theCyclin B1-Cdk1complex is activated, it is not deactivated.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
891
Immunogen:
Human partial recombinant protein (AA 1-433) was used as the immunogen for this Cyclin B1 antibody.
Limitation:
This Cyclin B1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human