Cyclin D1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30349
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30349-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the Cyclin D1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Cyclin D1 antibody and Lane 1: rat testis; 2: human placenta; 3: rat brain; 4: MCF-7; 5: COLO320; 6: SW620; 7: MM231. Predicted molecular weight: 32-36 kDa.~
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Flow cytometry testing of human U-87 MG cells with Cyclin D1 anti
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Western blot testing of Cyclin D1 antibody and Lane 1: rat testis; 2: human placenta; 3: rat brain; 4: MCF-7; 5: COLO320; 6: SW620; 7: MM231. Predicted molecular weight: 32-36 kDa.~
Flow cytometry testing of human U-87 MG cells with Cyclin D1 anti

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the Cyclin D1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cyclin D1, also known as CCND1, belongs to the highly conserved cyclin family whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of the protein plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The gene is overexpressed in breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al. (2003) found that prolactin (PRL; 176760) induced IGF2 (147470) mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. They also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and Cyclin D1 are components of a developmental pathway in mammary gland.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 19-37 (DANLLNDRVLRAMLKAEET) were used as the immunogen for this Cyclin D1 antibody.
Limitation:
This Cyclin D1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
P24385