Cyclophilin A Antibody

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NSJ Bioreagents
Product Code: NSJ-R32747
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32747-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cyclophilin A antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC testing of FFPE human intestinal cancer tissue with Cyclophilin A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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IHC testing of FFPE mouse intestine tissue with Cyclophilin A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 9
IHC testing of FFPE rat spleen tissue with Cyclophilin A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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Western blot testing of 1) rat lung, 2) rat brain, 3) mouse lung and 4) human HeLa lysate with Cyclophilin A antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
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Flow cytometry testing of human U937 cells with Cyclophilin A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Cyclophilin A antibody.
6 / 9
Flow cytometry testing of human K562 cells with Cyclophilin A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Cyclophilin A antibody.
7 / 9
Flow cytometry testing of human THP-1 cells with Cyclophilin A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Cyclophilin A antibody.
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IF/ICC staining of FFPE human A549 cells with Cyclophilin A antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human ThP-1 cells with Cyclophilin A antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
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IHC testing of FFPE human intestinal cancer tissue with Cyclophilin A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse intestine tissue with Cyclophilin A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat spleen tissue with Cyclophilin A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of 1) rat lung, 2) rat brain, 3) mouse lung and 4) human HeLa lysate with Cyclophilin A antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
Flow cytometry testing of human U937 cells with Cyclophilin A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Cyclophilin A antibody.
Flow cytometry testing of human K562 cells with Cyclophilin A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Cyclophilin A antibody.
Flow cytometry testing of human THP-1 cells with Cyclophilin A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Cyclophilin A antibody.
IF/ICC staining of FFPE human A549 cells with Cyclophilin A antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human ThP-1 cells with Cyclophilin A antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence/Immunocytochemistry: 2-4ug/ml
Application Note:
Optimal dilution of the Cyclophilin A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Cyclophilin A (PPIA), Peptidylprolyl isomerase A, is an enzyme that in humans is encoded by the PPIA gene. Using chromosome 7 and chromosome 10 deletion hybrid panels, the PPIA coding gene is localized to 7p13-p11.2. This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. Cyclophilin A is also a member of the immunophilin class of proteins that all possess peptidyl-prolyl cis/trans isomerase activity and are believed to be involved in protein folding and/or intracellular protein transport. And Cyclophilin A binds to the Gag protein of human immunodeficiency virus type 1 (HIV-1). Additionally, Cyclophilin A may have an essential function in HIV-1 replication.may have an essential function in HIV-1 replication.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids T116-E165 from the human protein were used as the immunogen for the Cyclophilin A antibody.
Limitation:
This Cyclophilin A antibody is available for research use only.
Localization:
Cytoplasmic, secreted
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P62937