CYPOR Antibody

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NSJ Bioreagents
Product Code: NSJ-R31055
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31055-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CYPOR antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of CYPOR antibody and rat samples: 1. ovary; 2. lung; 3. testis; 4. spleen; and human samples 5. A549; 6. HeLa; 7. SKOV; 8. MCF-7 cell lysate. Predicted/observed size ~77KD
2 / 4
IHC-P: CYPOR antibody testing of human placenta tissue. HIER: steamed with pH6 citrate buffer.
3 / 4
ICC testing of CYPOR antibody and human placenta cells
4 / 4
IHC staining of FFPE rat ovary with CYPOR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Western blot testing of CYPOR antibody and rat samples: 1. ovary; 2. lung; 3. testis; 4. spleen; and human samples 5. A549; 6. HeLa; 7. SKOV; 8. MCF-7 cell lysate. Predicted/observed size ~77KD
IHC-P: CYPOR antibody testing of human placenta tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of CYPOR antibody and human placenta cells
IHC staining of FFPE rat ovary with CYPOR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CYPOR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cytochrome P450 Oxidoreductase (CYPOR), also known as Cytochrome P450 Reductase, is a membrane-bound enzyme required for electron transfer to cytochrome P450 in the endoplasmic reticulumof the eukaryotic cell from a FAD- and FMN-containing enzyme NADPH: cytochrome P450 reductase. By Southern blot analysis of DNA isolated from a panel of 8 independent human-rodent somatic cell hybrids, Shephard et al.(1989) determined that CYPOR is encoded by a single gene located on 7pter-q22. By in situ hybridization to metaphase chromosomes, they refined the localization to 7q11.2. Cytochrome P450 oxidoreductase is a flavoprotein that donates electrons to all microsomal P450 enzymes, including the steroidogenic enzymes P450c17 (CYP17A1), P450c21 (CYP21A2), and CYP51A1 (Miller, 1986).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Cytochrome P450 Oxidoreductase (DYIKKLMTKGRYSLDVWS) was used as the immunogen for this CYPOR antibody.
Limitation:
This CYPOR antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P16435