Cystathionase Antibody (Cystathionine gamma lyase)

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NSJ Bioreagents
Product Code: NSJ-R30633
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30633-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cystathionase antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A431 cells with Cystathionase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human K562, 2) human HepG2, 3) human ThP-1, 4) human HCCT, 5) rat liver and 6) mouse liver tissue lysate with Cystathionase antibody. Predicted molecular weight ~44 kDa.
3 / 3
Flow cytometry testing of human JK cells with Cystathionase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cystathionase antibody.
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Immunofluorescent staining of FFPE human A431 cells with Cystathionase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human K562, 2) human HepG2, 3) human ThP-1, 4) human HCCT, 5) rat liver and 6) mouse liver tissue lysate with Cystathionase antibody. Predicted molecular weight ~44 kDa.
Flow cytometry testing of human JK cells with Cystathionase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cystathionase antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Cystathionase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cystathionine gamma lyase (or Cystathionase) is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. The International Radiation Hybrid Mapping Consortium mapped the CTH gene to chromosome 1. The gene had earlier been assigned to chromosome 16 by study of somatic cell hybrids. It is demonstrated that hydrogen sulfide(H2S) is physiologically generated by CTH.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Cystathionine gamma lyase (FYIKGTLQHAEIFLKN) was used as the immunogen for this Cystathionase antibody.
Limitation:
This Cystathionase antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P32929