Cytochrome C Antibody

NSJ Bioreagents
Product Code: NSJ-R31747
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31747-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cytochrome C antibody at 40. After reconstitution, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Cytochrome C antibody and Lane 1: rat brain; 2: mouse brain; 3: rat heart; 4: mouse heart; 5: U87; 6: Neuro-2a; 7: HeLa; 8: Jurkat; 9: human placenta lysate
2 / 8
IHC-P: Cytochrome C antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
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ICC testing of Cytochrome C antibody and SMMC-7721 cells
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IHC-P testing of mouse brain tissue. HIER: steamed with pH6 citrate buffer.
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IHC-P testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
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Immunofluorescent staining of FFPE human intestinal cancer tissue with Cytochrome C antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE mouse heart tissue with Cytochrome C antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE rat heart tissue with Cytochrome C antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of Cytochrome C antibody and Lane 1: rat brain; 2: mouse brain; 3: rat heart; 4: mouse heart; 5: U87; 6: Neuro-2a; 7: HeLa; 8: Jurkat; 9: human placenta lysate
IHC-P: Cytochrome C antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of Cytochrome C antibody and SMMC-7721 cells
IHC-P testing of mouse brain tissue. HIER: steamed with pH6 citrate buffer.
IHC-P testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
Immunofluorescent staining of FFPE human intestinal cancer tissue with Cytochrome C antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE mouse heart tissue with Cytochrome C antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat heart tissue with Cytochrome C antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence (FFPE): 1-2ug/ml
Application Note:
Titration of the Cytochrome C antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
54205
Immunogen:
Human partial recombinant protein (AA 2-105) was used as the immunogen for this Cytochrome C antibody.
Limitation:
This Cytochrome C antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat