Cytokeratin 14 Antibody (CK14, KRT14)

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NSJ Bioreagents
Product Code: NSJ-R32606
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32606-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cytokeratin 14 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of 1) rat brain, 2) mouse NIH3T3 and 3) human HepG2 lysate with Cytokeratin 14 antibody at 0.5ug/ml. Predicted molecular weight ~53 kDa.
2 / 6
IHC testing of FFPE human tonsil tissue with Cytokeratin 14 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 6
IHC testing of FFPE human esophageal squamous cancer with Cytokeratin 14 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 6
IHC testing of FFPE human esophageal squamous cancer with Cytokeratin 14 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
5 / 6
Flow cytometry testing of human U-2 OS cells with Cytokeratin 14 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytokeratin 14 antibody.
6 / 6
Flow cytometry testing of human SiHa cells with Cytokeratin 14 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytokeratin 14 antibody.
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Western blot testing of 1) rat brain, 2) mouse NIH3T3 and 3) human HepG2 lysate with Cytokeratin 14 antibody at 0.5ug/ml. Predicted molecular weight ~53 kDa.
IHC testing of FFPE human tonsil tissue with Cytokeratin 14 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human esophageal squamous cancer with Cytokeratin 14 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human esophageal squamous cancer with Cytokeratin 14 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-2 OS cells with Cytokeratin 14 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytokeratin 14 antibody.
Flow cytometry testing of human SiHa cells with Cytokeratin 14 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytokeratin 14 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Cytokeratin 14 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Cytokeratin 14, also known as cytokeratin-14 (CK-14) or keratin-14 (KRT14), is a member of the type I keratin family of intermediate filament proteins. In humans it is encoded by the KRT14 gene. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 446-472 (RQIRTKVMDVHDGKVVSTHEQVLRTKN-human) were used as the immunogen for the Cytokeratin 14 antibody.
Limitation:
This antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P02533