Cytokeratin 18 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30024
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30024-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: CY-90, aka CK-18
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cytokeratin 18 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human rectal cancer tissue with Cytokeratin 18 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 4
IHC staining of FFPE human breast cancer tissue with Cytokeratin 18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE human breast cancer tissue with Cytokeratin 18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 4
Western blot testing of human 1) placenta, 2) A431, 3) Caco-2 and 4) HepG2 cell lysate with Cytokeratin 18 antibody. Predicted molecular weight ~48 kDa.
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Immunofluorescent staining of FFPE human rectal cancer tissue with Cytokeratin 18 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human breast cancer tissue with Cytokeratin 18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with Cytokeratin 18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) placenta, 2) A431, 3) Caco-2 and 4) HepG2 cell lysate with Cytokeratin 18 antibody. Predicted molecular weight ~48 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,IHC (Paraffin): 1-2ug/ml,Immunohistochemistry (Frozen): 0.5-2ug/ml; formalin or acetone fixed tissues
Application Note:
The stated application concentrations are suggested starting points. Titration of the Cytokeratin 18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18 (KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the Cytokeratin 18/Keratin 18 protein is coded for by 7 exons. By Southern blotting using the genomic DNA PCR product, the gene for Cytokeratin 18 has been assigned to chromosome 12. Mutation of human Keratin 18 has been associated with cryptogenic cirrhosis.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
3875
Immunogen:
The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines was used as the immunogen for this Cytokeratin 18 antibody.
Limitation:
This Cytokeratin 18 antibody is available for research use only.
Purity:
Unpurified ascites
Species Reactivity :
Human, rat