Cytokeratin 19 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30400
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30400-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cytokeratin 19 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Cytokeratin 19 antibody and Lane 1: HT1080; 2: COLO320 cell lysate
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IHC-P: Cytokeratin 19 antibody testing of human oesophagus squama cancer tissue
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ICC testing of MCF-7 cells
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Western blot testing of Cytokeratin 19 antibody and Lane 1: HT1080; 2: COLO320 cell lysate
IHC-P: Cytokeratin 19 antibody testing of human oesophagus squama cancer tissue
ICC testing of MCF-7 cells

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Cytokeratin 19 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Keratin, type I cytoskeletal 19 is a protein that in humans is encoded by the KRT19 gene.The protein encoded by this gene is a member of the keratin family. It is specifically expressed in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. Keratin 19 is often used together with keratin 8 and keratin 18 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Cytokeratin 19 (YRSLLEGQEDHYNN) was used as the immunogen for this Cytokeratin 19 antibody.
Limitation:
This Cytokeratin 19 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P08727