Daxx Antibody

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NSJ Bioreagents
Product Code: NSJ-R32346
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32346-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Daxx antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human 293T cells with Daxx antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Daxx antibody.
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IHC testing of FFPE human intestinal cancer tissue with Daxx antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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IHC testing of FFPE rat intestine with Daxx antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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ICC testing of human SMMC-7721 cells with Daxx antibody.
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ICC testing of human A549 cells with Daxx antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Daxx antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of 1) human K562, 2) rat PC-12 and 3) mouse thymus lysate with Daxx antibody. Predicted molecular weight ~81 kDa but routinely observed at ~120 kDa, the slow SDS-PAGE migration possibly due to the proteins high acidic residue content.
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Flow cytometry testing of human 293T cells with Daxx antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Daxx antibody.
IHC testing of FFPE human intestinal cancer tissue with Daxx antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat intestine with Daxx antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
ICC testing of human SMMC-7721 cells with Daxx antibody.
ICC testing of human A549 cells with Daxx antibody.
Immunofluorescent staining of FFPE human U-2 OS cells with Daxx antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human K562, 2) rat PC-12 and 3) mouse thymus lysate with Daxx antibody. Predicted molecular weight ~81 kDa but routinely observed at ~120 kDa, the slow SDS-PAGE migration possibly due to the proteins high acidic residue content.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Daxx antibody should be determined by the researcher.
Description:
DAXX (death-domain associated protein) also known as DAP6 (Death-associated protein 6) or BING2, was first discovered through its cytoplasmic interaction with the classical death receptor Fas. Human DAXX encodes a 740-amino acid polypeptide containing a nuclear localization signal. Functional analyses by Yang et al. (1997) demonstrated that Daxx binds to the Fas death domain and enhances Fas-mediated apoptosis. The authors suggested that DAXX and FADD define 2 distinct apoptotic pathways downstream of Fas. The DAXX gene is mapped to human chromosome 6p21.3 by somatic cell hybrid panels and fluorescence in situ hybridization, a region containing the HLA and putative autoimmune disease genes. MSP58 overexpression relieved DAXX-mediated transcriptional repression. Immunoprecipitation and Western blot analysis with DAXX mutants showed that the N terminus of DAXX interacts with the C terminus of DMAP. Transient expression of DAXX or DMAP1 caused repression of glucocorticoid receptor-mediated transcription.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 56-345 of human Daxx were used as the immunogen for the Daxx antibody.
Limitation:
This Daxx antibody is available for research use only.
Localization:
Nuclear and cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
Q9UER7