DBI Antibody / Diazepam Binding Inhibitor

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NSJ Bioreagents
Product Code: NSJ-R32604
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32604-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the DBI antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human placenta lysate with DBI antibody at 0.5ug/ml. Predicted molecular weight ~10 kDa.
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IHC testing of FFPE human placenta tissue with DBI antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
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IHC testing of FFPE human prostate cancer with DBI antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human MCF7 cells with DBI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=DBI antibody.
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Flow cytometry testing of human PC-3 cells with DBI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=DBI antibody.
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Flow cytometry testing of human ThP-1 cells with DBI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=DBI antibody.
7 / 7
IF/ICC staining of FFPE human U-2 OS cells with DBI antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of human placenta lysate with DBI antibody at 0.5ug/ml. Predicted molecular weight ~10 kDa.
IHC testing of FFPE human placenta tissue with DBI antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human prostate cancer with DBI antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human MCF7 cells with DBI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=DBI antibody.
Flow cytometry testing of human PC-3 cells with DBI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=DBI antibody.
Flow cytometry testing of human ThP-1 cells with DBI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=DBI antibody.
IF/ICC staining of FFPE human U-2 OS cells with DBI antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence/Immunocytochemistry: 2-4ug/ml
Application Note:
Optimal dilution of the DBI antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Acyl-CoA-binding protein, also called Diazepam binding inhibitor, is a protein that in humans is encoded by the DBI gene. This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein corresponding to amino acids S2-I87 was used as the immunogen for the DBI antibody.
Limitation:
This DBI antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P07108