DCI Antibody

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NSJ Bioreagents
Product Code: NSJ-R30856
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30856-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the DCI antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of DCI antibody and Lane 1: rat liver; 2: human placenta; 3: A549; 4: SMMC-7721; 5: COLO320; 6: HeLa; 7: HT1080 cell lysate. Predicted molecular weight ~33 kDa.
2 / 6
IHC-P: DCI antibody testing of human breast cancer tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 6
ICC testing of HeLa cells. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 6
ICC testing of A549 cells. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 6
IF/ICC staining of FFPE human A549 cells with DCI antibody (red) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
6 / 6
IF/ICC staining of FFPE human A549 cells with DCI antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of DCI antibody and Lane 1: rat liver; 2: human placenta; 3: A549; 4: SMMC-7721; 5: COLO320; 6: HeLa; 7: HT1080 cell lysate. Predicted molecular weight ~33 kDa.
IHC-P: DCI antibody testing of human breast cancer tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
ICC testing of HeLa cells. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
ICC testing of A549 cells. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IF/ICC staining of FFPE human A549 cells with DCI antibody (red) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IF/ICC staining of FFPE human A549 cells with DCI antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry (FFPE): 0.5-1ug/ml, Immunofluorescence (FFPE): 2-4ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the DCI antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Dodecenoyl-CoA Delta Isomerase, also known as 3,2-trans-enoyl-CoA isomerase (ECI1), is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon(position 3) to trans double bonds at beta-carbon(position 2). It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase/DCI is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human DCI (ADVQNFVSFISKDSIQKSL) was used as the immunogen for this DCI antibody.
Limitation:
This DCI antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P42126