DCK Antibody / Deoxycytidine kinase

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NSJ Bioreagents
Product Code: NSJ-RQ4200
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4200-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the DCK antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of 1) mouse spleen, 2) mouse thymus and human 3) HeLa, 4) U-87 MG, 5) MCF7 and 6) U-2 OS lysate with DCK antibody at 0.5ug/ml. Predicted molecular weight ~30 kDa.
2 / 6
IHC staining of FFPE human lung cancer with DCK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 6
IHC staining of FFPE human intestinal cancer with DCK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 6
IHC staining of FFPE mouse small intestine with DCK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 6
IHC staining of FFPE rat small intestine with DCK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
6 / 6
Flow cytometry testing of human U-2 OS cells with DCK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DCK antibody.
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Western blot testing of 1) mouse spleen, 2) mouse thymus and human 3) HeLa, 4) U-87 MG, 5) MCF7 and 6) U-2 OS lysate with DCK antibody at 0.5ug/ml. Predicted molecular weight ~30 kDa.
IHC staining of FFPE human lung cancer with DCK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human intestinal cancer with DCK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE mouse small intestine with DCK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat small intestine with DCK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human U-2 OS cells with DCK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DCK antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DCK antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Deoxycytidine kinase (dCK) is an enzyme which is encoded by the DCK gene in humans. Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids E17-L260 was used as the immunogen for the DCK antibody.
Limitation:
This DCK antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P27707