DDB1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32243
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32243-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the DDB1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with DDB1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC testing of FFPE human intestinal cancer with DDB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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IHC testing of FFPE mouse liver with DDB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 12
IHC testing of FFPE rat liver with DDB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
5 / 12
IHC testing of frozen human placental tissue with DDB1 antibody.
6 / 12
ICC testing of FFPE human SMMC-7721 cells with DDB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
7 / 12
ICC testing of FFPE human PC-3 cells with DDB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
8 / 12
ICC testing of FFPE human A549 cells with DDB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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Western blot testing of 1) rat brain, 2) rat liver, 3) mouse ovary, 4) mouse testis and 5) human MCF7 lysate. Predicted/observed molecular weight ~127 kDa.
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Flow cytometry testing of human 293T cells with DDB1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDB1 antibody.
11 / 12
Western blot testing of human 1) HeLa, 2) HepG2, 3) HEK293, 4) K562 5) Raji, 6) Caco-2 and 7) ThP-1 cell lysate with DDB1 antibody. Predicted molecular weight ~127 kDa.
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Western blot testing of 1) rat brain, 2) rat kidney, 3) rat C6, 4) mouse brain, 5) mouse kidney and 6) mouse testis tissue lysate with DDB1 antibody. Predicted molecular weight ~127 kDa.
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Immunofluorescent staining of FFPE human A431 cells with DDB1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human intestinal cancer with DDB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse liver with DDB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat liver with DDB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen human placental tissue with DDB1 antibody.
ICC testing of FFPE human SMMC-7721 cells with DDB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
ICC testing of FFPE human PC-3 cells with DDB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
ICC testing of FFPE human A549 cells with DDB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Western blot testing of 1) rat brain, 2) rat liver, 3) mouse ovary, 4) mouse testis and 5) human MCF7 lysate. Predicted/observed molecular weight ~127 kDa.
Flow cytometry testing of human 293T cells with DDB1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDB1 antibody.
Western blot testing of human 1) HeLa, 2) HepG2, 3) HEK293, 4) K562 5) Raji, 6) Caco-2 and 7) ThP-1 cell lysate with DDB1 antibody. Predicted molecular weight ~127 kDa.
Western blot testing of 1) rat brain, 2) rat kidney, 3) rat C6, 4) mouse brain, 5) mouse kidney and 6) mouse testis tissue lysate with DDB1 antibody. Predicted molecular weight ~127 kDa.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the DDB1 antibody should be determined by the researcher.
Description:
The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. And this protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 1011-1140 of human DDB1 were used as the immunogen for the DDB1 antibody.
Limitation:
This DDB1 antibody is available for research use only.
Localization:
Nuclear and cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q16531