DDX5 Antibody

NSJ Bioreagents
Product Code: NSJ-R31068
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31068-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the DDX5 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of DDX5 antibody and Lane 1: rat testis; 2: mouse testis; 3: human HeLa; 4: (h) MCF-7; 5: (m) NIH3T3; 6: (h) SKOV; 7: (h) MM231. Predicted/observed size ~68KD~
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IHC-P: DDX5 antibody testing of rat brain tissue

Western blot testing of DDX5 antibody and Lane 1: rat testis; 2: mouse testis; 3: human HeLa; 4: (h) MCF-7; 5: (m) NIH3T3; 6: (h) SKOV; 7: (h) MM231. Predicted/observed size ~68KD~
IHC-P: DDX5 antibody testing of rat brain tissue

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the DDX5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
DEAD/H box 5, also known as p68, HLR1 or G17P1, is an enzyme that in humans is encoded by the DDX5 gene. It is a proliferation-associated nuclear antigen first identified through its highly specific cross-reaction with the simian virus 40 tumor antigen(Iggo et al., 1989). Subsequently, homology to eukaryotic translation initiation factor was found, and amino acid sequence blocks characteristic of a large superfamily of proteins with putative helicase activity were demonstrated. Brody et al.(1995) confirmed that this gene is located on chromosome 17 in the region of the BRCA1 gene at 17q21. By immunoprecipitation analysis, Caretti et al.(2006) found that p68 (DDX5), p72(DDX17), and the noncoding RNA SRA(SRA1) associated with MYOD (MYOD1) in MYOD-transfected HeLa cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human DEAD/H box 5 (TYQNGYDSTQQYGSN) was used as the immunogen for this DDX5 antibody (100% homologous in human, mouse and rat).
Limitation:
This DDX5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P17844