DNA Polymerase lambda Antibody / POLL

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NSJ Bioreagents
Product Code: NSJ-RQ4359
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4359-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the DNA Polymerase lambda antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) COLO-320, 3) MCF7 and 4) HepG2 cell lysate with DNA Polymerase lambda antibody at 0.5ug/ml. Predicted molecular weight ~63 kDa, also observed at ~68 kDa.
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Flow cytometry testing of human U-2 OS cells with DNA Polymerase lambda antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNA Polymerase lambda antibody.
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Western blot testing of human 1) HeLa, 2) COLO-320, 3) MCF7 and 4) HepG2 cell lysate with DNA Polymerase lambda antibody at 0.5ug/ml. Predicted molecular weight ~63 kDa, also observed at ~68 kDa.
Flow cytometry testing of human U-2 OS cells with DNA Polymerase lambda antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNA Polymerase lambda antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DNA Polymerase lambda antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
DNA polymerase lambda, also known as POLL, is a protein found in eukaryotes. In humans, it is encoded by the POLLA gene. POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, suggesting that POLL participates in short- and long-patch base excision repair. POLL is widely expressed, with abundant expression in pachytene spermatocytes of testis and in ovary.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids K307-N407 was used as the immunogen for the DNA Polymerase lambda antibody.
Limitation:
This DNA Polymerase lambda antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9UGP5