DR4 Antibody / Death Receptor 4

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NSJ Bioreagents
Product Code: NSJ-R32614
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32614-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the DR4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with DR4 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) rat spleen, 2) mouse spleen and 3) human MCF7 lysate with DR4 antibody at 0.5ug/ml. Predicted molecular weight ~50 kDa.
3 / 3
Flow cytometry testing of human A549 cells with DR4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DR4 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with DR4 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat spleen, 2) mouse spleen and 3) human MCF7 lysate with DR4 antibody at 0.5ug/ml. Predicted molecular weight ~50 kDa.
Flow cytometry testing of human A549 cells with DR4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DR4 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
Optimal dilution of the DR4 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
TNFRSF10A (Tumor Necrosis Factor Receptor Subfamily Member 10A), also known as APO2, DR4 or TRAILR1, is a protein that in humans is encoded by the TNFRSF10A gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 99-131 (VLLQVVPSSAATIKLHDQSIGTQQWEHSPLGEL-human) were used as the immunogen for the DR4 antibody..
Limitation:
This antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O00220